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Thermo Fisher
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer
doi: 10.3390/ijms21103466
Figure Lengend Snippet: Expression of triosephosphate isomerase. ( A ) In 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the protein migrates as at least 3 proteoforms (indicated with black circles) with varying isoelectric points but similar molecular masses that each vary in concentration. ( B ) When analyzed by 1D western blotting the proteoforms co-migrate as one band where the total concentration is similar in the two cell lines.
Article Snippet: Cell pellets were re-suspended in SDS sample buffer (LC2676,
Techniques: Expressing, Polyacrylamide Gel Electrophoresis, Concentration Assay, Western Blot